beta-Actin Antibody (AC-15), Novus Biologicals™

Catalog No.	Quantity
NB600501	0.1 mL

Catalog No. NB600501 Supplier Novus Biologicals Supplier No. NB600501

Description

Mouse Monoclonal Antibody has been used in 442 publications

beta-Actin Monoclonal specifically detects beta-Actin in Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Feline, Fish, Guinea Pig, Hamster, Leech, Mammal, Primate, Rabbit, Sheep, Squirrel, Turkey, Zebrafish, Drosophila (Negative) samples. It is validated for Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Knockdown Validated.

Specifications

• Antigen: beta-Actin
• Applications: ChIP Assay, ELISA, Flow Cytometry, Immunoassay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, KnockDown
• Classification: Monoclonal
• Clone: AC-15
• Conjugate: Unconjugated
• Dilution: Western Blot 1:5000 - 1:10000, Simple Western 1:25, Flow Cytometry, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:500, Chromatin Immunoprecipitation (ChIP), Knockout Validated, KnockDown Validated
• Gene Accession No.: P60709
• Gene Alias: ACTB, actin, beta, actin, cytoplasmic 1, anti-beta actin, B-Actin, Beta Actin, beta actin (ac-15), beta cytoskeletal actin, Beta-actin, beta-actin ac 15, beta-actin ac15, beta-actin ac-15, BRWS1, I(2)-Actin, PS1TP5-binding protein 1, PS1TP5BP1
• Gene Symbols: ACTB
• Host Species: Mouse
• Immunogen: This beta-Actin Antibody (AC-15) was made to a slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
• Molecular Weight of Antigen: 42 kDa
• Purification Method: Unpurified
• Quantity: 0.1 mL
• Regulatory Status: RUO
• Research Discipline: Angiogenesis, Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Extracellular Matrix, Loading Controls, Signal Transduction, Stem Cell Markers
• Primary or Secondary: Primary
• Gene ID (Entrez): 60
• Test Specificity: In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.
• Target Species: Human, Mouse, Rat, Pig, Bovine, Canine, Chicken, Feline, Fish, Guinea Pig, Hamster, Leech, Mammalia, Primate, Rabbit, Sheep, Squirrel, Turkey, Zebrafish, Drosophila (Negative)
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Form: Ascites
• Isotype: IgG1

For Research Use Only