beta-Actin Antibody (AC-15), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 442 publications

Supplier:  Novus Biologicals NB600501

Catalog No. NB600501

Description

beta-Actin Monoclonal specifically detects beta-Actin in Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Feline, Fish, Guinea Pig, Hamster, Leech, Mammal, Primate, Rabbit, Sheep, Squirrel, Turkey, Zebrafish, Drosophila (Negative) samples. It is validated for Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Knockdown Validated.

Specifications

• Antigen: beta-Actin
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene Accession No.: P60709
• Gene Symbols: ACTB
• Immunogen: This beta-Actin Antibody (AC-15) was made to a slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1

• Applications: ChIP Assay, ELISA, Flow Cytometry, Immunoassay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, KnockDown
• Clone: AC-15
• Dilution: Western Blot 1:5000 - 1:10000, Simple Western 1:25, Flow Cytometry, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:500, Chromatin Immunoprecipitation (ChIP), Knockout Validated, KnockDown Validated
• Gene Alias: ACTB, actin, beta, actin, cytoplasmic 1, anti-beta actin, B-Actin, Beta Actin, beta actin (ac-15), beta cytoskeletal actin, Beta-actin, beta-actin ac 15, beta-actin ac15, beta-actin ac-15, BRWS1, I(2)-Actin, PS1TP5-binding protein 1, PS1TP5BP1
• Host Species: Mouse
• Molecular Weight of Antigen: 42 kDa
• Quantity: 0.1 mL
• Research Discipline: Angiogenesis, Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Extracellular Matrix, Loading Controls, Signal Transduction, Stem Cell Markers
• Gene ID (Entrez): 60
• Target Species: Human, Mouse, Rat, Pig, Bovine, Canine, Chicken, Feline, Fish, Guinea Pig, Hamster, Leech, Mammalia, Primate, Rabbit, Sheep, Squirrel, Turkey, Zebrafish, Drosophila (Negative)
• Form: Ascites

For Research Use Only