beta-Actin Antibody (AC-15), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 442 publications

Catalog Number	Mfr. No.	Quantity
NB600501	Novus Biologicals NB600501	0.1 mL

Description

beta-Actin Monoclonal specifically detects beta-Actin in Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Feline, Fish, Guinea Pig, Hamster, Leech, Mammal, Primate, Rabbit, Sheep, Squirrel, Turkey, Zebrafish, Drosophila (Negative) samples. It is validated for Western Blot, Simple Western, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Knockdown Validated.

Specifications

• Antigen: beta-Actin
• Dilution: Western Blot 1:5000 - 1:10000, Simple Western 1:25, Flow Cytometry, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:1000 - 1:2000, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:500, Chromatin Immunoprecipitation (ChIP), Knockout Validated, KnockDown Validated
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Angiogenesis, Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Extracellular Matrix, Loading Controls, Signal Transduction, Stem Cell Markers
• Gene Alias: ACTB, actin, beta, actin, cytoplasmic 1, anti-beta actin, B-Actin, Beta Actin, beta actin (ac-15), beta cytoskeletal actin, Beta-actin, beta-actin ac 15, beta-actin ac15, beta-actin ac-15, BRWS1, I(2)-Actin, PS1TP5-binding protein 1, PS1TP5BP1
• Gene Symbols: ACTB
• Isotype: IgG1
• Purification Method: Unpurified
• Test Specificity: In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. The epitope recognized by the antibody is resistant to formalin-fixed and paraffin-embedding.

• Clone: AC-15
• Classification: Monoclonal
• Form: Ascites
• Regulatory Status: RUO
• Gene Accession No.: P60709
• Gene ID (Entrez): 60
• Immunogen: This beta-Actin Antibody (AC-15) was made to a slightly modified Beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 42 kDa

For Research Use Only