beta Actin Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 600401886S

Catalog No. 50-199-1843

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-beta-Actin Loading Control Antibody reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

Specifications

• Antigen: beta Actin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ACTB
• Gene Alias: ACT-5; ACTA1; ACTB; actb.L; actb1; actba; actin; actin beta; actin, alpha 1, skeletal muscle; actin, beta; actin, beta 1; actin, beta L homeolog; actin, beta, cytoplasmic; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Actx; Atcb; bact; bactin1; bactin1 protein; bactzf; B-ACTZF; beta actin; beta cytoskeletal actin; Beta-actin; Beta-actin-1; BOS_22932; BRWS1; Cap Z; capZ beta; cytoplasmic 1; Cytoplasmic beta-actin; cytoskeletal protein; cytoskeleton actin; E430023M04Rik; gamma actin; gamma non-muscle actin; PS1TP5-binding protein 1; PS1TP5BP1; RCJMB04_4h19; wu:fd18f05; XELAEV_18045052mg
• Host Species: Rabbit
• Regulatory Status: RUO
• Gene ID (Entrez): 100135470, 100689477, 101844587, 102179831, 11461, 280979, 414396, 443052, 60, 81822
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P48975, P60709, P60710, P60711, P60712, P60713, Q6QAQ1, Q71FK5
• Gene Symbols: ACTB
• Quantity: 25 μL
• Primary or Secondary: Primary
• Target Species: Bovine, Goat, Guinea Pig, Hamster, Human, Monkey, Mouse, Ovine, Pig, Rat
• Product Type: Antibody