Invitrogen™ betacellulin Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P254BT500UG

Catalog No. 50-271-8565

Description

AA Sequence of recombinant protein: DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Betacellulin. Anti-Human Betacellulin-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hBetacellulin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Betacellulin (500-P254) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBetacellulin. Western Blot: To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBetacellulin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P254BT-1MG will be provided as 2 x 500 μg

The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor.

Specifications

• Antigen: betacellulin
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: BTC
• Gene Alias: Bcn; betacellulin; betacellulin, epidermal growth factor family member; BTC; OTTHUMP00000160600; OTTHUMP00000219057; Probetacellulin
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 685
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P35070
• Gene Symbols: BTC
• Immunogen: E.coli-derived Recombinant Human Betacellulin.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG