Thermo Scientific™ BglII (10 U/μL)

Description

Cut at A^GATCT sites with BglII restriction enzyme, which performs best at 37°C in O buffer.

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

• Superior quality – stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern Blot
• Restriction fragment length polymorphism (RFLP)
• SNP

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Specifications

• Concentration: 10 U/μL
• Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
• Enzyme: Bgl II
• Compatible Buffer: 10X Buffer O
• Sensitive to Heat Inactivation: No
• Optimal Reaction Temperature: 37°C
• Type IIS RE: No
• Quantity: 2,500 units
• Product Type: Restriction Enzyme
• Research Category: Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.