Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/2032R) - Azide and BSA Free, Novus Biologicals™

Rabbit Monoclonal Antibody

Supplier:  Novus Biologicals NBP261393200UG

Catalog No. NB261393200

Description

Biotin (Vitamin B7 or Vitamin H) Monoclonal antibody specifically detects Biotin (Vitamin B7 or Vitamin H) in All Species samples. It is validated for Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, In-situ Hybridization.

Specifications

• Antigen: Biotin (Vitamin B7 or Vitamin H)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Dilution: Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence 0.5-1 ug/ml, Immunohistochemistry-Paraffin 0.5-1 ug/ml, In-situ Hybridization
• Host Species: Mouse
• Molecular Weight of Antigen: 2.4 kDa
• Quantity: 200 μg
• Test Specificity: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), In Situ Hybridization (ISH)
• Clone: BTN/2032R
• Conjugate: Unconjugated
• Formulation: 10mM PBS - BSA free with No Preservative
• Immunogen: Biotinylated sheep immunoglobulin
• Purification Method: Protein A or G purified
• Primary or Secondary: Primary
• Target Species: All species
• Form: Purified