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Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/2032R), Novus Biologicals™
SDP

Catalog No. NB261392200 Shop All R&D Systems Products
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Catalog No. Quantity
NB261392200 200 μg
NBP26139220 20 μg
NB261392100 100 μg
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Catalog No. NB261392200 Supplier Novus Biologicals Supplier No. NBP261392200UG

Description

Rabbit Monoclonal Antibody

Biotin (Vitamin B7 or Vitamin H) Monoclonal antibody specifically detects Biotin (Vitamin B7 or Vitamin H) in All Species samples. It is validated for Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, In-situ Hybridization.

Specifications

Antigen Biotin (Vitamin B7 or Vitamin H)
Applications Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), In Situ Hybridization (ISH)
Classification Monoclonal
Clone BTN/2032R
Conjugate Unconjugated
Dilution Western Blot, Flow Cytometry 0.5-1 ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1 ug/ml, Immunohistochemistry-Paraffin 0.5-1 ug/ml, In-situ Hybridization
Formulation Prepared in 10mM PBS with 0.05% BSA with 0.05% Sodium Azide
Host Species Mouse
Immunogen Biotinylated sheep immunoglobulin
Molecular Weight of Antigen 2.4 kDa
Purification Method Protein A or G purified
Quantity 200 μg
Primary or Secondary Primary
Test Specificity It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Target Species All species
Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1
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