Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/2032R), Novus Biologicals™

Rabbit Monoclonal Antibody

Supplier:  Novus Biologicals NBP26139220UG

Catalog No. NBP26139220

Description

Biotin (Vitamin B7 or Vitamin H) Monoclonal specifically detects Biotin (Vitamin B7 or Vitamin H) in All Species samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin, In-situ Hybridization.

Specifications

• Antigen: Biotin (Vitamin B7 or Vitamin H)
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Prepared in 10mM PBS with 0.05% BSA with 0.05% Sodium Azide
• Immunogen: Biotinylated sheep immunoglobulin
• Purification Method: Protein A or G purified
• Primary or Secondary: Primary
• Target Species: All species
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), In Situ Hybridization (ISH)
• Clone: BTN/2032R
• Dilution: Western Blot, Flow Cytometry 0.5-1 ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1 ug/ml, Immunohistochemistry-Paraffin 0.5-1 ug/ml, In-situ Hybridization
• Host Species: Mouse
• Molecular Weight of Antigen: 2.4 kDa
• Quantity: 20 μg
• Test Specificity: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1