Invitrogen™ BRD4 Recombinant Rabbit Monoclonal Antibody (BL-149-2H5)

Rabbit Recombinant Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
PIMA544203	Invitrogen™ MA544203	100 μL
PIMA544204	Invitrogen™ MA544204	20 μL

Description

Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains. BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. BRD4 acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. BRD4 functions as a cellular target for E2 protein of papillomavirus.

Specifications

• Antigen: BRD4
• Concentration: 100 μg/mL
• Applications: ChIP sequencing (ChIP-seq), Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Rabbit
• Regulatory Status: RUO
• Formulation: PBS with 0.1% BSA and 0.09% sodium azide; pH 8.2
• Gene Accession No.: O60885
• Gene ID (Entrez): 23476
• Immunogen: Synthetic peptide representing a region between residues 1312 and 1362 of human bromodomain-containing protein 4.
• Product Type: Antibody

• Clone: BL-149-2H5
• Content And Storage: 4°C
• Classification: Recombinant Monoclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Human
• Gene: BRD4
• Gene Alias: Brd4; BRD4-NUT FUSION; Brd5; bromodomain containing 4; bromodomain-containing 4; bromodomain-containing 5; bromodomain-containing protein 4; bromodomain-containing protein 4; LOW QUALITY PROTEIN: bromodomain-containing protein 4; CAP; chromosome-associated protein; fsh-like protein; HUNK1; HUNKI; Mcap; mitotic chromosome-associated protein; Protein HUNK1; WI-11513
• Gene Symbols: BRD4
• Primary or Secondary: Primary
• Purification Method: Purified