Bromodeoxyuridine/BrdU Antibody (85-2C8), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2452220.1MG

Catalog No. NBP24522201

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Bromodeoxyuridine/BrdU Monoclonal specifically detects Bromodeoxyuridine/BrdU in All Species samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: Bromodeoxyuridine/BrdU
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Alias: Bromodeoxyuridine, BUdr
• Immunogen: Bromodeoxyuridine (BrdU) conjugated to hemocyanine (isolated from Helix pomatia)
• Quantity: 0.1 mg
• Primary or Secondary: Primary
• Target Species: Mouse, All species
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: 85-2C8
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Test Specificity: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
• Content And Storage: Store at 4C.
• Isotype: IgG1

For Research Use Only