Cytiva Butyl Sepharose™ 4 Fast Flow Chromatography Media

Description

Used for capture and intermediate purification of larger proteins

• Based on Sepharose Fast Flow base matrix derivatized via uncharged, chemically-stable ether linkages
• Hydrophobic interaction chromatography (HIC) media composed of cross-linked 4% agarose
• Aliphatic butyl ligand immobilized to base matrix with ether linkage
• Fast Flow matrix gives the adsorbent high chemical, physical and thermal stabilities
• Dynamic capacity at low salt concentration
• Used with all aqueous buffers commonly used in chromatography
• Autoclavable at 121°C for 20 minutes in water
• Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development

Specifications

• Quantity: 200 mL
• For Use With (Application): For Capture and Intermediate PurifiCatIon of Larger Proteins
• Stability: Stable in commonly used aqueous Buffers: 1 M NaOH, 30% Isopropanol, 70% Ethanol, 6 M Guanidine-Hydrochloride, 30% Acetonitrile, 1 mM HCl
• Storage Requirements: 4°C to 30°C
• Product Type: Butyl Sepharose 4 Fast Flow Media
• Flow Rate: 150 to 250 cm/Hoμr
• Particle Size: 45 to 165 μm
• Height (English) Bed: 9.84 in.
• Height (Metric) Bed: 25 cm
• Max. Pressure: 100 kPa
• Matrix: Based on cross-linked 4% agarose
• pH Range: 3 to 13
• Storage Buffer: 20% Ethanol
• Ligand Type: Butyl