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  5. Cytiva Butyl Sepharose™ 4 Fast Flow Chromatography Media

Cytiva Butyl Sepharose™ 4 Fast Flow Chromatography Media

Catalog No. 45002943 Shop All Cytiva Products
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200 mL
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45-002-943 200 mL
45-002-944 25 mL
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Catalog No. 45-002-943 Supplier Cytiva Supplier No. 17098001
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Description

Used for capture and intermediate purification of larger proteins

  • Based on Sepharose Fast Flow base matrix derivatized via uncharged, chemically-stable ether linkages
  • Hydrophobic interaction chromatography (HIC) media composed of cross-linked 4% agarose
  • Aliphatic butyl ligand immobilized to base matrix with ether linkage
  • Fast Flow matrix gives the adsorbent high chemical, physical and thermal stabilities
  • Dynamic capacity at low salt concentration
  • Used with all aqueous buffers commonly used in chromatography
  • Autoclavable at 121°C for 20 minutes in water
  • Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development

TRUSTED_SUSTAINABILITY

Specifications

Quantity 200 mL
For Use With (Application) For Capture and Intermediate PurifiCatIon of Larger Proteins
Stability Stable in commonly used aqueous Buffers: 1 M NaOH, 30% Isopropanol, 70% Ethanol, 6 M Guanidine-Hydrochloride, 30% Acetonitrile, 1 mM HCl
Storage Requirements 4°C to 30°C
Product Type Butyl Sepharose 4 Fast Flow Media
Flow Rate 150 to 250 cm/Hoμr
Particle Size 45 to 165 μm
Height (English) Bed 9.84 in.
Height (Metric) Bed 25 cm
Max. Pressure 100 kPa
Matrix Based on cross-linked 4% agarose
pH Range 3 to 13
Storage Buffer 20% Ethanol
Ligand Type Butyl
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