c-Myb, p Thr486 Antibody, Novus Biologicals™

Catalog No.	Quantity
NBP221659	0.1 mg

Catalog No. NBP221659 Supplier Novus Biologicals Supplier No. NBP221659

Description

Rabbit Polyclonal Antibody

c-Myb Polyclonal specifically detects c-Myb in Mouse samples. It is validated for Western Blot, ELISA.

Specifications

• Antigen: c-Myb (p Thr486)
• Applications: Western Blot, ELISA
• Classification: Polyclonal
• Concentration: 0.91 mg/ml
• Conjugate: Unconjugated
• Dilution: Western Blot 1:2500, ELISA 1:5000-1:16000
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Accession No.: P06876
• Gene Alias: c-myb, Cmyb, c-myb protein (140 AA), c-myb_CDS, c-myb10A_CDS, c-myb13A_CDS, c-myb14A_CDS, c-myb8B_CDS, efg, Proto-oncogene c-Myb, transcriptional activator Myb, v-myb avian myeloblastosis viral oncogene homolog, v-myb myeloblastosis viral oncogene homolog (avian)
• Gene Symbols: MYB
• Host Species: Rabbit
• Immunogen: This affinity purified c-Myb [p Thr486] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse c-Myb protein surrounding amino acid residue 486. (Uniprot: P06876)
• Molecular Weight of Antigen: 71.42 kDa
• Purification Method: Affinity Purified
• Quantity: 0.1 mg
• Regulatory Status: RUO
• Research Discipline: Cancer, Phospho Specific
• Primary or Secondary: Primary
• Gene ID (Entrez): 4602
• Test Specificity: This affinity-purified antibody is directed against the phosphorylated form of mouse c-Myb protein at the pT486 residue. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against mouse c-Myb pT486 protein and the antibody is specific for the phosphorylated form of the protein. This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. Reactivity with non-phosphorylated mouse c-Myb is minimal by ELISA and western blot. A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence. Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee; with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human; and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence. Cross-reactivity with c-Myb from other sources has not been determined.
• Target Species: Mouse
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
• Isotype: IgG

For Research Use Only