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Caldesmon/CALD1 Antibody (h-CALD) - Azide and BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody
Supplier: Novus Biologicals NBP2478160.1MG
Description
Caldesmon/CALD1 Monoclonal specifically detects Caldesmon/CALD1 in Human samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.Specifications
Caldesmon/CALD1 | |
Monoclonal | |
1.0 mg/mL | |
Simple Western : 10 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 1 - 2 ug/ml, CyTOF-ready | |
CAD, caldesmon, caldesmon 1, CDMH-CAD, HCAD, LCAD, L-CAD, MGC21352, NAG22 | |
Mouse | |
150 kDa | |
0.1 mg | |
Cytoskeleton Markers | |
800 | |
Human | |
Purified |
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF | |
h-CALD | |
Unconjugated | |
PBS with No Preservative | |
CALD1 | |
Crude human uterus extract | |
Protein A or G purified | |
RUO | |
Primary | |
Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. | |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | |
IgG1 κ |
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