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  6. Carbonic Anhydrase IX/CA9 Mouse, Clone: SPM487, Novus Biologicals™

Carbonic Anhydrase IX/CA9 Mouse, Clone: SPM487, Novus Biologicals™
SDP

Catalog No. NBP234363A Shop All R&D Systems Products
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NBP234363A 0.1 mg
NBP234363F 0.02 mg
NBP234363B 0.2 mg
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Catalog No. NBP234363A Supplier Novus Biologicals Supplier No. NBP2343630.1MG
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Description

Mouse Monoclonal Antibody

Carbonic Anhydrase IX/CA9 Monoclonal specifically detects Carbonic Anhydrase IX/CA9 in Human, Equine samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

Antigen Carbonic Anhydrase IX/CA9
Applications Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Classification Monoclonal
Clone SPM487
Conjugate Unconjugated
Dilution Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Formulation PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Accession No. Q16790
Gene Alias CA-IX, CAIXcarbonic anhydrase 9, Carbonate dehydratase IX, carbonic anhydrase IXpMW1, carbonic dehydratase, EC 4.2.1.1, G250, Membrane antigen MN, MNRCC-associated antigen G250, P54/58N, RCC-associated protein G250, Renal cell carcinoma-associated antigen G250
Gene Symbols CA9
Host Species Mouse
Immunogen Microsomal fraction of human renal cortical tissue homogenate
Molecular Weight of Antigen 200 kDa
Purification Method Protein A purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Cancer, Cellular Markers, HIF Target Genes, Hypoxia, Lipid and Metabolism, Signal Transduction, Vision
Primary or Secondary Primary
Gene ID (Entrez) 768
Test Specificity Recognizes a glycoprotein of ∼200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.
Target Species Human, Equine
Content And Storage Store at 4C.
Form Purified
Isotype IgG2b κ
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