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Caspase-3 Antibody - (active/cleaved), Novus Biologicals™
SDP

Catalog No. NB10056113 Shop All R&D Systems Products
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NB10056113 0.05 mL
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Catalog No. NB10056113 Supplier Novus Biologicals Supplier No. NB10056113
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Description

Rabbit Polyclonal Antibody has been used in 48 publications

Caspase-3 Polyclonal specifically detects Caspase-3 in Human, Mouse, Rat, Gerbil samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Flow (Intracellular).

Specifications

Antigen Caspase-3
Applications Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot
Classification Polyclonal
Conjugate Unconjugated
Dilution Western Blot 1:1000-1:2000, Flow Cytometry reported in scientific literature (PMID 28287161), Immunohistochemistry, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 29963272), Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000, Immunohistochemistry-Frozen reported in scientific literature (PMID 29864441), Immunoblotting reported in scientific literature (PMID 27912032), Flow (Intracellular) reported in scientific literature (PMID 24804954)
Formulation Neat whole antisera with 0.05% Sodium Azide
Gene Alias Apopain, apoptosis-related cysteine protease, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama
Gene Symbols CASP3
Host Species Rabbit
Immunogen This Caspase-3 Antibody - (active/cleaved) was developed against catalytically active human caspase-3 protein.
Molecular Weight of Antigen 31.7 kDa
Purification Method Unpurified
Quantity 0.05 mL
Regulatory Status RUO
Research Discipline Apoptosis, Cancer, Caspases, Phospho Specific
Primary or Secondary Primary
Gene ID (Entrez) 836
Test Specificity Preferentially detects active caspase-3 (large subunit: ∼14-21 kDa, and small subunit: ∼10 kDa). However, it may also detect pro-caspase-3 (∼32 kDa) in some cell or tissue systems. Nuclear immunostaining of caspase-3 is considered be an indication of active/cleaved caspase-3.
Target Species Human, Mouse, Rat, Gerbil
Content And Storage Store at -20C. Avoid freeze-thaw cycles.
Form Antisera
Isotype IgG
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For Research Use Only

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