Caspase-3 Antibody - (active/cleaved), Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 48 publications

Supplier:  Novus Biologicals NB10056113

Catalog No. NB10056113

Description

Caspase-3 Polyclonal specifically detects Caspase-3 in Human, Mouse, Rat, Gerbil samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Flow (Intracellular).

Specifications

• Antigen: Caspase-3
• Classification: Polyclonal
• Dilution: Western Blot 1:1000-1:2000, Flow Cytometry reported in scientific literature (PMID 28287161), Immunohistochemistry, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 29963272), Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000, Immunohistochemistry-Frozen reported in scientific literature (PMID 29864441), Immunoblotting reported in scientific literature (PMID 27912032), Flow (Intracellular) reported in scientific literature (PMID 24804954)
• Gene Alias: Apopain, apoptosis-related cysteine protease, CASP-3, caspase 3, apoptosis-related cysteine peptidase, caspase-3, CPP-32, CPP32B, CPP32SREBP cleavage activity 1, Cysteine protease CPP32, EC 3.4.22, EC 3.4.22.56, PARP cleavage protease, procaspase3, Protein Yama, SCA-1, Yama
• Host Species: Rabbit
• Molecular Weight of Antigen: 31.7 kDa
• Quantity: 0.05 mL
• Research Discipline: Apoptosis, Cancer, Caspases, Phospho Specific
• Gene ID (Entrez): 836
• Target Species: Human, Mouse, Rat, Gerbil
• Form: Antisera

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot
• Conjugate: Unconjugated
• Formulation: Neat whole antisera with 0.05% Sodium Azide
• Gene Symbols: CASP3
• Immunogen: This Caspase-3 Antibody - (active/cleaved) was developed against catalytically active human caspase-3 protein.
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Preferentially detects active caspase-3 (large subunit: ∼14-21 kDa, and small subunit: ∼10 kDa). However, it may also detect pro-caspase-3 (∼32 kDa) in some cell or tissue systems. Nuclear immunostaining of caspase-3 is considered be an indication of active/cleaved caspase-3.
• Content And Storage: Store at -20C. Avoid freeze-thaw cycles.
• Isotype: IgG

For Research Use Only