Promega Caspase-Glo™ 3/7 Assay Kit

Description

Includes

Caspase-Glo 3/7 Substrate; Caspase-Glo 3/7 Buffer

Caspase-Glo™ 3/7 Assay is a homogeneous luminescent assay that measures caspase-3/7 activities

Utilizes a proluminescent Caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a single reagent optimized for Caspase-3/7 activity, luciferase activity, and cell lysis. Stabilized luciferase improves assay performance across a wide range of assay conditions and makes the assay less likely to be affected by compound interference than other luminescent-, fluorescent-, or colorimetric-based assays.

• Designed for use with 96- or 384-well plates using either purified enzyme or cells in culture as sample
• Requires less enzyme or fewer cells than other caspase formats due to low background luminescence
• Requires no sample preparation, manipulation, or extended incubation times—maximum sensitivity is achieved in as little as one-half to one hour
• “Add-mix-measure” protocol makes the assay easy to automate
• Extended-glow signal allows the plates to be read over a three-hour period for batch processing—no injectors required

• Patent pending.
• The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patent Nos. 5,583,024; 5,674,713; and 5,700,673.

Specifications

• Quantity: 10 x 100 mL
• pH: 7.4
• Content And Storage: -20°C
• For Use With (Application): 96- or 384-well plates using either purified enzyme or cells in culture as sample
• Form: Liquid