Invitrogen™ CCL27 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P294BT500UG

Catalog No. 50-271-8534

Description

AA Sequence of recombinant protein: FLLPPSTACC TQLYRKPLSD KLLRKVIQVE LQEADGDCHL QAFVLHLAQR SICIHPQNPS LSQWFEHQER KLHGTLPKLN FGMLRKMG. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CTACK (CCL27). Anti-Human CTACK (CCL27)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human CTACK by sandwich ELISA (using 100 mL/well) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti- Human CTACK (500-P294) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CTACK. Western Blot: To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human CTACK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P294BT-1MG will be provided as 2 x 500 μg

CCL27 gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The protein encoded by this gene is chemotactic for skin-associated memory T lymphocytes. This cytokine may also play a role in mediating homing of lymphocytes to cutaneous sites. It specifically binds to chemokine receptor 10 (CCR10). Studies of a similar murine protein indicate that these protein-receptor interactions have a pivotal role in T cell-mediated skin inflammation.

Specifications

• Antigen: CCL27
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: CCL27
• Gene Alias: ALP; AW558992; CC chemokine ILC; C-C motif chemokine 27; C-C motif chemokine ligand 27; Ccl27; Ccl27a; chemokine (C-C motif) ligand 27; chemokine (C-C motif) ligand 27A; CTACK; CTAK; cutaneous T-cell attracting chemokine; cutaneous T-cell-attracting chemokine; ESkine; IL-11 R-alpha-locus chemokine; IL-11 Ralpha-locus chemokine; ILC; PESKY; SCYA27; Scya27a; Skinkine; skinskine; small inducible cytokine A27; small inducible cytokine A27a; small inducible cytokine subfamily A (Cys-Cys), member 27; small-inducible cytokine A27
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10850
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: Q9Y4X3
• Gene Symbols: CCL27
• Immunogen: E.coli-derived Recombinant Human CTACK (CCL27).
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG