CD11c Mouse anti-Human, Clone-ITGAX/1242, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP254431200UG

Catalog No. NB254431200

Description

IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes

Specifications

• Antigen: CD11c
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells, Immunocytochemistry/Immunofluorescence 0.5 - 1 ug/ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, CyTOF-ready
• Gene Alias: 95, 95 alpha chain, 95, alpha subunit, CD11 antigen-like family member C, CD11c, CD11c antigen, CD11Cleu M5, alpha subunit, integrin alpha-X, integrin, alpha X (antigen CD11C (p150), alpha polypeptide), integrin, alpha X (complement component 3 receptor 4 subunit), Leu M5, Leukocyte adhesion glycoprotein p150, Leukocyte adhesion receptor p150, leukocyte surface antigen p150, myeloid membrane antigen, alpha subunit, p150 95 integrin alpha chain, SLEB6
• Host Species: Mouse
• Molecular Weight of Antigen: 145 kDa
• Quantity: 200 μg
• Research Discipline: Dendritic Cell Markers, Signal Transduction
• Gene ID (Entrez): 3687
• Target Species: Human
• Isotype: IgG κ

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), CyTOF
• Clone: ITGAX/1242
• Conjugate: Unconjugated
• Formulation: PBS with No Preservative
• Gene Symbols: CD11c
• Immunogen: Recombinant fragment (191 Amino acid residues between aa 600-850) of human ITGAX protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 145kDa, identified as CD11c. CD11c (ITGAX), a member of the leukointegrin family, shares the same beta subunit with other members of the leukocyte adhesion molecule family, which includes CD11a (LFA-1), CD11b (MAC-1) and CD11d (ITGAD), but has a unique alpha chain. CD11c has been shown to play a role in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T-cell proliferation by Langerhans cells. CD11c is expressed prominently on the plasma membranes of monocytes, tissue macrophages, NK cells, and most dendritic cells (DCs). A lower level of expression is also observed on neutrophils as a result of its high level of expression on most DCs. An antibody to CD11c may aid in identification of lesions with histiocytic origin. It may also been used as a marker for hairy cell leukemia in paraffin-embedded tissues.
• Content And Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.