CD120a (TNF Receptor I) Monoclonal Antibody (55R-170), Functional Grade, eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-144-38	Invitrogen 16120281	50 μg
50-144-39	Invitrogen 16120285	500 μg

Description

Description: The 55R-170 monoclonal antibody reacts with mouse Tumor Necrosis Factor Receptor I (TNFRI, TNFR-p55, TNFRSF1A, CD120a). TNFRI is expressed in a variety of cell types. The soluble TNF receptors are truncated forms of cell surface receptors with neutralizing activity on both TNF-alpha and TNF-beta. It has been suggested that the proinflammatory and immunosuppressive properties of TNF segregate at the level of its receptors and that the pool of TNF bound to soluble receptors could represent a reservoir to control the TNF release. Applications Reported: The 55R-170 antibody has been reported for use in ELISA, neutralization of mouse TNFR I bioactivity and flow cytometric analysis. Applications Tested: The Functional Grade Purified 55R-170 antibody has been tested by LAL assay to verify low endotoxin levels and in bioassay for neutralization of mouse TNFR I bioactivity. The 55R-170 antibody at 25 μg/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse TNF alpha in an assay of TNF alpha induced cytotoxicity of L929 cells. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the Best Protocols™ section. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE.

TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily, and is one of the major TNF-alpha receptors. TNFR1 plays an important role in mediating, in cytokine mediated signaling, positive regulation of the NF-Kb pathway, positive regulation of angiogenesis, and negative regulation of gene expression. The extracellular domain of TNFR1 is also released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12. Anti-apoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with TNFR1, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of TNFR1 were found to be associated with the autosomal dominant periodic fever syndrome, and the impaired receptor clearance is thought to be a mechanism of the disease.

Specifications

• Antigen: CD120a (TNF Receptor I)
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene Alias: CD120a; CD120a antigen; FPF; hypothetical protein; metalloproteinase inhibitor 4; MGC19588; MS5; n-smase activation assoc. factor; p55; p55 TNF receptor; p55-R; p60; p62; p63; sCD120a; soluble CD120a; soluble TNFR1; sTNF RI; sTNFR I; sTNFR1; sTNFRI; TBP1; TBPI; TIMP metallopeptidase inhibitor 4; Timp4; TIMP-4; tissue inhibitor of metalloproteinase 4; tissue inhibitor of metalloproteinases 4; TNF receptor alpha chain; TNF receptor superfamily member 1A; TNF-alphaR1; TNF-alpha-R1; TNFalpha-R1; TNFAR; TNFR; TNF-R; TNFR I; TNFR1; TNF-R1; Tnfr-1; TNFR1-d2; Tnfr-2; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRp55; Tnfrsf1a; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor; tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor superfamily member 1A, membrane form; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor; Tumor necrosis factor-binding protein 1
• Gene Symbols: Tnfrsf1a
• Primary or Secondary: Primary
• Content And Storage: 4° C
• Gene: Tnfrsf1a

• Clone: 55R-170
• Applications: Functional Assay, Neutralization
• Conjugate: Functional Grade
• Host Species: Armenian Hamster
• Target Species: Mouse
• Gene Accession No.: P25118
• Gene ID (Entrez): 21937
• Isotype: IgG
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.