Invitrogen™ CD120b (TNF Receptor II) Monoclonal Antibody (TR75-54), eBioscience™, Invitrogen™

Armenian Hamster Monoclonal Antibody

Supplier:  Invitrogen™ 14120385

Catalog No. 50-128-37

Description

Description: The TR75-54 monoclonal antibody reacts with mouse Tumor Necrosis Factor Receptor II (TNFRII, TNFR-p80, TNFRSF1B, CD120b). TNFRII is expressed in a variety of cell types and strongly expressed on stimulated T and B lymphocytes. TNFRII is the main TNF receptor found on circulating T cells and is the major mediator of autoregulatory apoptosis in CD8+ cells. The soluble TNF receptors are truncated forms of cell surface receptors with neutralizing activity on both TNF-alpha and TNF-beta. It has been suggested that the proinflammatory and immunosuppressive properties of TNF segregate at the level of its receptors and that the pool of TNF bound to soluble receptors could represent a reservoir to control the TNF release. Applications Reported: This TR75-54 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and ELISA. Applications Tested: The TR75-54 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse TNFRII in combination with the biotin TR75-32 (cat. 13-1204) antibody for detection and recombit mouse TNFRII as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 μg/mL. A standard curve consisting of doubling dilutions of the recombit standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. Purity: Greater than 90%, as determined by SDS-PAGE.

TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha. It has a much lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.

Specifications

• Antigen: CD120b (TNF Receptor II)
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P25119
• Gene Symbols: Tnfrsf1b
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 21938
• Content And Storage: 4°C
• Form: Liquid

• Applications: ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Clone: TR75-54
• Conjugate: Unconjugated
• Gene: Tnfrsf1b
• Gene Alias: CD120b; Etanercept; p75; p75 TNF receptor; p75 TNFR; p75TNFR; p80 TNF-alpha receptor; sCD120b; soluble CD120b; soluble TNFR1B variant 1; soluble TNFR2; sTNF R2; sTNFR 2; sTNFR2; TBP-2; TBPII; TNF receptor beta chain; TNF receptor superfamily member 1B; TNF-alphaR2; TNFalpha-R2; TNFBR; TNFR; TNFR II; Tnfr-1; TNFR1B; TNFR2; TNF-R2; Tnfr-2; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor; tumor necrosis factor receptor 2; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor superfamily member 1b, membrane form; tumor necrosis factor receptor superfamily, member 1B; tumor necrosis factor receptor type II; Tumor necrosis factor-binding protein 2
• Host Species: Armenian Hamster
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG

For Research Use Only.