CD141 Monoclonal Antibody (LS17-9), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
14-141-137	Invitrogen™ 14141137	2 mg

Description

The MAb LS17-9 recognizes mouse CD141 (Thrombomodulin, BDCA-3), a type I cell surface glycoprotein that binds to and allosterically activates thrombin. This complex cleaves protein C and the Thrombin Activatable Fibrinolysis Inhibitor (TAFI), initiating an important anticoagulant and antifibrinolytic pathway. Although expressed across many tissues and cell types, its highest expression has been reported on the lumenal surface of vascular endothelial cell. In the immune system the highest expression can be observed on monocytes and macrophages. Soluble CD141 is constitutively present in the serum at a very low level, and can be upregulated during inflammation. The anti-inflammatory activity of CD141 results from its ability to block the highly pro-inflammatory activity of thrombin. CD141 prevents chemotaxis and binding of granulocytes to activated endothelium, and inhibits activation of eosinophils and mast cells thereby reducing inflammation. Although human CD141 is commonly used as a marker of cross-presenting dendritic cells, mouse CD141 does not exhibit the same expression pattern.

Thrombomodulin (TM) is also known as Fetomodulin (FM), endothelial anticoagulant protein, or glycoprotein P112. Thrombomodulin contains six repeated domains homologous with epidermal growth factor (EGF) and an amino terminal domain homologous to lectin-like protein. Through its accelerated activation of protein C, synthesis of TM is one of several mechanisms important in maintaining thrombo-resistance and thus reducing clot formation on the surface of the endothelial cells. Immunohistochemical staining of formalin-fixed tissue for TM has been used for the study of a variety of vascular tumors, meningiomas and choriocarcinomas.

Specifications

• Antigen: CD141
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: P15306
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: LS17-9
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene ID (Entrez): 21824
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: THBD