MilliporeSigma™ CD152 (CTLA-4), Mouse, PE, Clone: BNI3,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABF1622

Catalog No. MABF1622

Description

The BNI3 antibody is specific for human CD152, commonly known as CTLA-4, a 33-37kDa protein expressed as a homodimer on the surface of activated T and B cells, and on thymocytes. CTLA-4 is structurally similar, yet functionally disparate, to the T cell co-stimulatory molecule CD28. Both CTLA-4 and CD28 interact with the co-stimulatory molecules CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells, with CTLA-4 displaying a higher avidity than CD28. While CD28 typically delivers a potent co-stimulatory signal in support of T cell activation, CTLA-4 appears to act as a negative regulator of T cell activation and may contribute to the suppressor function of Treg cells.

CTLA-4 proteins may be initially sequestered within Golgi vesicles, from which they can be transferred to and from the cell surface, a mechanism by which Treg cells can selectively impart suppressive functions. The BNI3 antibody may be used for flow cytometric analysis of intracellular or surface CTLA-4 expression, and is also widely used for neutralization of CTLA-4 when expressed at the cell surface. The BNI3 antibody is reported to be cross-reactive with Baboon, Cynomolgus and Rhesus CTLA-4. Please choose the appropriate format for each application.

Specifications

• Antigen: CD152 (CTLA-4)
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified mouse monoclonal IgG2a in buffer containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
• Gene Accession No.: P16410
• Immunogen: Immunogen information for this antibody is proprietary information.
• Quantity: 100 Tests
• Research Discipline: Inflammation & Immunology
• Target Species: Human
• Form: Purified

• Applications: Flow Cytometry
• Clone: BNI3
• Conjugate: PE
• Gene: CTLA4, CD152
• Host Species: Mouse
• Purification Method: Purified by size-exclusion chromatography
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
• Isotype: IgG2a

For Research Use Only. Not for use in diagnostic procedures.