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CD20 Antibody (B9E9), Alexa Fluor™ 350, Novus Biologicals™
SDP

Catalog No. NP2349A30 Shop All R&D Systems Products
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NP2349A30 0.1 mL
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Catalog No. NP2349A30 Supplier Novus Biologicals Supplier No. NBP234559AF350
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Description

Mouse Monoclonal Antibody

CD20 Monoclonal specifically detects CD20 in Human samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence.

Specifications

Antigen CD20
Applications Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
Classification Monoclonal
Clone B9E9
Conjugate Alexa Fluor 350
Dilution Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Frozen
Formulation 50 mM sodium borate with 0.05% sodium azide
Gene Alias B1, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, B-lymphocyte surface antigen B1, Bp35MGC3969, CD20 antigen, CD20 receptor, CD20S7, CVID5, LEU-16, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, membrane-spanning 4-domains, subfamily A, member 1, MS4A2
Gene Symbols MS4A1
Host Species Mouse
Immunogen Lymphoblastoid cell line Daudi.
Purification Method Protein A or G purified
Quantity 0.1 mL
Research Discipline Adaptive Immunity, B Cell Development and Differentiation Markers, Cancer, Cancer Stem Cells, Cell Biology, Cytokine Research, Immunology, Signal Transduction, Stem Cell Markers, Tumor Biomarkers
Primary or Secondary Primary
Gene ID (Entrez) 931
Test Specificity Recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This monoclonal antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
Target Species Human
Content And Storage Store at 4°C in the dark.
Isotype IgG2a κ
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