CD20 Antibody (B9E9), PE/Cy5.5, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP234559PECY55

Catalog No. N23459PECY5

Description

CD20 Monoclonal specifically detects CD20 in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD20
• Classification: Monoclonal
• Conjugate: PE-Cyanine5.5
• Formulation: PBS with 0.05% Sodium Azide
• Gene Symbols: MS4A1
• Immunogen: Lymphoblastoid cell line Daudi.
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This monoclonal antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
• Content And Storage: Store at 4°C in the dark. Do not freeze.

• Applications: Flow Cytometry
• Clone: B9E9
• Dilution: Flow Cytometry
• Gene Alias: B1, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, B-lymphocyte surface antigen B1, Bp35MGC3969, CD20 antigen, CD20 receptor, CD20S7, CVID5, LEU-16, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, membrane-spanning 4-domains, subfamily A, member 1, MS4A2
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Research Discipline: Adaptive Immunity, B Cell Development and Differentiation Markers, Cancer, Cancer Stem Cells, Cell Biology, Cytokine Research, Immunology, Signal Transduction, Stem Cell Markers, Tumor Biomarkers
• Gene ID (Entrez): 931.0
• Target Species: Human
• Isotype: IgG2a κ