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CD20 Antibody (L26 + IGEL/773), Novus Biologicals™
SDP

Catalog No. NBP24474701 Shop All R&D Systems Products
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NBP24474701 0.1 mL
NBP24474705 0.5 mL
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Catalog No. NBP24474701 Supplier Novus Biologicals Supplier No. NBP2447470.1ML
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

CD20 Monoclonal specifically detects CD20 in Human, Baboon, Monkey, Bovine (Negative), Canine (Negative), Porcine (Negative), Rat (Negative) samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

Antigen CD20
Applications Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Classification Monoclonal
Clone L26 + IGEL/773
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry 5 - 10 ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1:100-1:200, Immunofluorescence 1:50 - 1:100
Formulation No buffer with 0.05% Sodium Azide
Gene Accession No. P11836
Gene Alias B1, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, B-lymphocyte surface antigen B1, Bp35MGC3969, CD20 antigen, CD20 receptor, CD20S7, CVID5, LEU-16, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, membrane-spanning 4-domains, subfamily A, member 1, MS4A2
Gene Symbols MS4A1
Host Species Mouse
Immunogen Human tonsil B cells (L26); Recombinant human MS4A1 protein (IGEL/773)
Molecular Weight of Antigen 35 kDa
Purification Method Tissue culture supernatant
Quantity 0.1 mL
Regulatory Status RUO
Research Discipline Adaptive Immunity, Cancer Stem Cells, Cell Biology, Cytokine Research, Immunology, Signal Transduction, Stem Cell Markers, Tumor Biomarkers
Primary or Secondary Primary
Gene ID (Entrez) 931
Test Specificity Recognizes a protein of 30-33kDa, which is identified as CD20. Its epitope is located in the cytoplasmic domain of CD20 and was, therefore, ascribed as CD20cy in the 5th Workshop. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkins disease, particularly of lymphocyte predominant type.
Target Species Human, Baboon, Monkey, Bovine (Negative), Canine (Negative), Porcine (Negative), Rat (Negative)
Content And Storage Store at 4C.
Form Supernatant
Isotype IgG
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