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CD25/IL-2R alpha Antibody (143-13), Novus Biologicals™
SDP

Catalog No. NBP24508800 Shop All R&D Systems Products
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NBP24508800 0.02 mg
NBP24508801 0.1 mg
NBP24508802 0.2 mg
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Catalog No. NBP24508800 Supplier Novus Biologicals Supplier No. NBP2450880.02MG
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

CD25/IL-2R alpha Monoclonal specifically detects CD25/IL-2R alpha in Human samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Functional, Immunofluorescence.

Specifications

Antigen CD25/IL-2 R alpha
Applications Flow Cytometry, SDS-Page, Immunofluorescence
Classification Monoclonal
Clone 143-13
Concentration 0.2 mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Accession No. P01589, P01589
Gene Alias CD25, CD25 antigen, IDDM10, IL-2 receptor subunit alpha, IL2R, IL-2R subunit alpha, IL-2-RA, IL2-RA, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR
Gene Symbols IL2RA
Host Species Mouse
Immunogen Stimulated human leukocytes
Molecular Weight of Antigen 55 kDa
Purification Method Protein A or G purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Adaptive Immunity, Apoptosis, Cytokine Research, Diabetes Research, Immunology, Innate Immunity, Mast Cell Markers, Signal Transduction
Primary or Secondary Primary
Gene ID (Entrez) 3559
Test Specificity Recognizes a protein of 55kDa, identified as CD25. It is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin's disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and doe not block IL-2 binding to CD25.
Target Species Human
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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For Research Use Only

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