CD25/IL-2R alpha Antibody (Basiliximab) - Chimeric - Azide and BSA Free, Novus Biologicals™

Rabbit Monoclonal Antibody

Supplier:  Novus Biologicals NBP2526610.025MG

Catalog No. NBP25266125

Description

CD25/IL-2R alpha Monoclonal specifically detects CD25/IL-2R alpha in Human, Cynomolgus Monkey, Rhesus Macaque samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunofluorescence, CyTOF-ready, Block/Neutralize.

Specifications

• Antigen: CD25/IL-2 R alpha
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: PBS with 0.02% Proclin 300. with 0.02% Proclin 300
• Gene Symbols: IL2RA
• Immunogen: CD25
• Quantity: 0.025 mg
• Research Discipline: Adaptive Immunity, Apoptosis, B Cell Development and Differentiation Markers, Cytokine Research, Diabetes Research, Immunology, Innate Immunity, Mast Cell Markers, Signal Transduction
• Gene ID (Entrez): 3559
• Target Species: Human, Mouse, Rat
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Blocking Assay, Immunofluorescence, CyTOF
• Clone: Basiliximab
• Dilution: Western Blot 1:100 - 1:2000, Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 1:10 - 1:500, Blocking/Neutralizing 50 - 200 molar excess, Immunofluorescence 1:10 - 1:500, CyTOF-ready
• Gene Alias: CD25, CD25 antigen, IDDM10, IL-2 receptor subunit alpha, IL2R, IL-2R subunit alpha, IL-2-RA, IL2-RA, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR
• Host Species: Rabbit
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit - binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R ( alpha beta yc) - T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG