CD301b (MGL2) Monoclonal Antibody (11A10-B7), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
14-301-182	Invitrogen™ 14301182	100 μg
14-301-137	Invitrogen™ 14301137	2 mg

Description

Description: The monoclonal antibody 11A10-B7 recognizes mouse CD301b. CD301b is also known as macrophage galactose-type C-type lectin 2 (MGL2). CD301b expression is restricted to dermal dendritic cells, connection tissue, and bone marrow derived dendritic cells. Macrophages also express CD301b at low levels. CD301b contains a single carbohydrate recognition domain that recognizes protein antigens that have a galactose and N-acetylgalactosamine (Gal/GalNAc). Gal/GalNAc protein antigens are efficiently internalized through CD301b and presented to antigen specific CD4+ cells via MHC Class II. CD301b+ dendritic cells are believed to play an important role in TH2 priming. Applications Reported: This 11A10-B7 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, and microscopy. Applications Tested: This 11A10-B7 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

CD301b is also known as macrophage galactose-type C-type lectin 2 (MGL2). CD301b expression is restricted to dermal dendritic cells, connection tissue, and bone marrow derived dendritic cells. Macrophages also express CD301b at low levels. CD301b contains a single carbohydrate recognition domain that recognizes protein antigens that have a galactose and N-acetylgalactosamine (Gal/GalNAc). Gal/GalNAc protein antigens are efficiently internalized through CD301b and presented to antigen specific CD4+ cells via MHC Class II. CD301b+ dendritic cells are believed to play an important role in TH2 priming.

Specifications

• Antigen: CD301b (MGL2)
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Host Species: Rat
• Target Species: Mouse
• Gene Alias: CD301; CD301b; macrophage galactose N-acetyl-galactosamine specific lectin 2; macrophage galactose-type C-type lectin 2; Mgl2
• Isotype: IgG2a λ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: 11A10-B7
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene ID (Entrez): 216864
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: Mgl2