CD35 Antibody (E11), Novus Biologicals™

Catalog No.	Quantity
NBP229460	0.1 mg

Catalog No. NBP229460 Supplier Novus Biologicals Supplier No. NBP229460

Description

Mouse Monoclonal Antibody has been used in 5 publications

CD35 Monoclonal specifically detects CD35 in Baboon, Cynomolgus Monkey, Human, Monkey, Rhesus Macaque samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), In Situ Hybridization (ISH), In-situ Hybridization.

Specifications

• Antigen: CD35
• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin), In Situ Hybridization (ISH), In Situ Hybridization (ISH)
• Classification: Monoclonal
• Clone: E11
• Concentration: 0.2 mg/ml
• Conjugate: Unconjugated
• Dilution: Flow Cytometry 0.5-1 μg/million cells, Immunohistochemistry 0.5-1 μg/mL, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunohistochemistry-Paraffin 0.5-1 μg/mL, In-situ Hybridization
• Gene Accession No.: P17927
• Gene Alias: complement component (3b/4b) receptor 1 (Knops blood group), KN
• Gene Symbols: CR1
• Host Species: Mouse
• Immunogen: Intact human monocytes were used as immunogen to generate the CD35 antibody (Hogg et al, 1984).
• Molecular Weight of Antigen: 215 kDa
• Purification Method: Protein A or G purified
• Quantity: 0.1 mg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1378
• Test Specificity: Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This monoclonal antibody is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This monoclonal antibody is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This monoclonal antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.
• Target Species: Baboon, Cynomolgus Monkey, Human, Monkey, Rhesus Macaque
• Content And Storage: Store at 4C.
• Form: Purified
• Isotype: IgG1 κ

For Research Use Only