CD35 Antibody (E11), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 5 publications

Supplier:  Novus Biologicals NBP229460

Catalog No. NBP229460

Description

CD35 Monoclonal specifically detects CD35 in Human, Monkey, Baboon, Cynomolgus Monkey, Rhesus Macaque samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: CD35
• Classification: Monoclonal
• Concentration: 0.2 mg/ml
• Dilution: Flow Cytometry 0.5-1 μg/million cells, Immunohistochemistry 0.5-1 μg/mL, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunohistochemistry-Paraffin 0.5-1 μg/mL, In-situ Hybridization
• Gene Alias: complement component (3b/4b) receptor 1 (Knops blood group), KN
• Host Species: Mouse
• Molecular Weight of Antigen: 215 kDa
• Quantity: 0.1 mg
• Primary or Secondary: Primary
• Test Specificity: Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This monoclonal antibody is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This monoclonal antibody is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This monoclonal antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), In Situ Hybridization (ISH), Immunoprecipitation
• Clone: E11
• Conjugate: Unconjugated
• Gene Accession No.: P17927
• Gene Symbols: CR1
• Immunogen: Intact human monocytes were used as immunogen to generate the CD35 antibody (Hogg et al, 1984).
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Gene ID (Entrez): 1378
• Target Species: Human, Monkey
• Form: Purified

For Research Use Only