CD41a Monoclonal Antibody (HIP8), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-3051	LIFE TECHNOLOGIES 17041942	100 Tests

Description

Description: The HIP8 monoclonal antibody reacts with the human CD41 molecule, the integrin alpha IIb also known as platelet GPIIb. CD41 non-covalently associates with integrin beta3 (GPIIIa, CD61) and is expressed by megakaryocytes and platelets. The CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin and mediates platelets aggregation. HIP8 blocks platelet aggregation. Applications Reported: This HIP8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIP8 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD41 (platelet glycoprotein IIb, ITGA2B) is composed of two subunits -120 kDa a, alpha and 23 kDa b, beta- that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. CD41 is also involved in blood coagulation by mediating platelet aggregation. Upon blood vessel damage, CD41 binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during the early stages of hematopoietic differentiation. Diseases associated with CD41 dysfunction include Glansmann Thrombasthenia, and Platelet type-16 bleeding disorder.

Specifications

• Antigen: CD41a
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P08514
• Isotype: IgG1 κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody

• Clone: HIP8
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Mouse
• Target Species: Human
• Gene ID (Entrez): 3674
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!

For Research Use Only.