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  6. CD45RA Antibody (PTPRC/1148) - Azide and BSA Free, Novus Biologicals™

CD45RA Antibody (PTPRC/1148) - Azide and BSA Free, Novus Biologicals™
SDP

Catalog No. NB254335200 Shop All R&D Systems Products
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NB254335200 200 μg
NB254335100 100 μg
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Catalog No. NB254335200 Supplier Novus Biologicals Supplier No. NBP254335200UG

Description

Mouse Monoclonal Antibody

CD45RA Monoclonal antibody specifically detects CD45RA in Human samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, CyTOF-ready.

Specifications

Antigen CD45RA
Applications Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), CyTOF
Classification Monoclonal
Clone PTPRC/1148
Concentration 1 mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry 0.5 - 1 ug/million cells, Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, CyTOF-ready
Formulation PBS with No Preservative
Gene Alias B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbols PTPRC
Host Species Mouse
Immunogen Recombinant full-length human CD45RA protein
Molecular Weight of Antigen 213 kDa
Purification Method Protein A or G purified
Quantity 200 μg
Regulatory Status RUO
Research Discipline Adaptive Immunity, Cell Biology, Cellular Markers, Cellular Signaling, Cytokine Research, Glia Markers, Hematopoietic Stem Cell Markers, Immunology, Immunophenotyping, MAP Kinase Signaling, Mast Cell Markers, Mesenchymal Stem Cell Markers, Microglia Markers, Modulation of DNA Pools, Myeloid Cell Markers, Myeloid derived Suppressor Cell, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers
Primary or Secondary Primary
Gene ID (Entrez) 5788
Test Specificity Recognizes a protein of 205kDa-220kDa, identified as CD45RA. It is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
Target Species Human
Content And Storage Store at -20 to -80C. Avoid freeze-thaw cycles.
Form Purified
Isotype IgG1 κ
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