CD5 Antibody (C5/473 + CD5/54/F6), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP234316F	Novus Biologicals NBP2343160.02MG	0.02 mg
NBP234316A	Novus Biologicals NBP2343160.1MG	0.1 mg
NBP234316B	Novus Biologicals NBP2343160.2MG	0.2 mg

Description

CD5 Monoclonal specifically detects CD5 in Human, Rat, Bovine, Rabbit samples. It is validated for ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: CD5
• Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Accession No.: P06127
• Gene ID (Entrez): 921
• Immunogen: Human CD5 recombinant protein (C5/473); A synthetic peptide from the intracellular region of human CD5 (CD5/54/F6)
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 67 kDa

• Clone: C5/473 + CD5/54/F6
• Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Cell Biology, Cytokine Research, Immunology
• Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
• Gene Alias: CD5 antigen, CD5 antigen (p56-62), CD5 molecule, LEU1T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, T1
• Gene Symbols: CD5
• Isotype: IgG
• Purification Method: Protein A purified
• Test Specificity: Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (∼10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.