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CD55/DAF Antibody (143-30), Novus Biologicals™
SDP

Catalog No. NBP24487301 Shop All R&D Systems Products
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NBP24487301 0.1 mg
NBP24487300 0.02 mg
NBP24487302 0.2 mg
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Catalog No. NBP24487301 Supplier Novus Biologicals Supplier No. NBP2448730.1MG
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence

CD55/DAF Monoclonal specifically detects CD55/DAF in Human samples. It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Flow (Cell Surface).

Specifications

Antigen CD55/DAF
Applications Flow Cytometry, Immunohistochemistry (Frozen), SDS-Page, Immunofluorescence
Classification Monoclonal
Clone 143-30
Concentration 0.2 mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Accession No. P08174, P08174
Gene Alias CD55 antigen, CD55 molecule, decay accelerating factor for complement (Cromer blood group), CRdecay accelerating factor for complement (CD55, Cromer blood group system), CROMDAFcomplement decay-accelerating factor, decay accelerating factor for complement, TC
Gene Symbols CD55
Host Species Mouse
Immunogen PHA stimulated human PBL
Molecular Weight of Antigen 70 kDa
Purification Method Protein A or G purified
Quantity 0.1 mg
Regulatory Status RUO
Research Discipline Immunology
Primary or Secondary Primary
Gene ID (Entrez) 1604
Test Specificity Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
Target Species Human
Content And Storage Store at 4C.
Form Purified
Isotype IgG1 κ
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For Research Use Only

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