CD55/DAF Antibody (143-30), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2448730.2MG

Catalog No. NBP24487302

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence

CD55/DAF Monoclonal antibody specifically detects CD55/DAF in Human samples. It is validated for Flow Cytometry, Immunohistochemistry-Frozen, SDS-Page, Flow (Cell Surface), Immunofluorescence.

Specifications

• Antigen: CD55/DAF
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen 0.5 - 1.0 ug/ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Accession No.: P08174, P08174
• Gene Symbols: CD55
• Immunogen: PHA stimulated human PBL
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), SDS-Page, Immunofluorescence
• Clone: 143-30
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: CD55 antigen, CD55 molecule, decay accelerating factor for complement (Cromer blood group), CRdecay accelerating factor for complement (CD55, Cromer blood group system), CROMDAFcomplement decay-accelerating factor, decay accelerating factor for complement, TC
• Host Species: Mouse
• Molecular Weight of Antigen: 70 kDa
• Quantity: 0.2 mg
• Research Discipline: Immunology
• Gene ID (Entrez): 1604
• Target Species: Human
• Form: Purified

For Research Use Only