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Invitrogen™ CD57 Monoclonal Antibody (TB01 (TBO1)), PE-Cyanine7, eBioscience™

Description
Description: This TB01 monoclonal antibody reacts with human CD57 (also known as HNK-1 and Leu-7), a 110-kDa cell surface glycoprotein expressed on a subset of natural killer (NK) cells and NK T cells. Applications Reported: This TB01 (TBO1) antibody has been reported for use in flow cytometric analysis. Applications Tested: This TB01 (TBO1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (1.0 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.
Specifications
Specifications
| Antigen | CD57 |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | TB01 (TBO1) |
| Concentration | 5 μL/Test |
| Conjugate | PE-Cyanine7 |
| Formulation | PBS with BSA and 0.09% sodium azide; pH 7.2 |
| Gene | B3GAT1 |
| Gene Accession No. | Q9P2W7 |
| Gene Alias | HNK-1; Leu-7 |
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