Invitrogen™ CD61 (Integrin beta 3) Monoclonal Antibody (VI-PL2), Super Bright™ 645, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 64061942

Catalog No. 64-061-942

Description

Description: The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation. Applications Reported: This H1.2F3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This may be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No.

CD61, also known as GPIIIa or ITGB3, is a 105 kDa glycoprotein expressed on activated T cells, granulocytes, megakaryocytes, platelets, and their precursors. It plays a crucial role in platelet aggregation and functions as a receptor for fibrinogen, fibronectin, von Willebrand factor, vitronectin, and thrombospondin. CD61 forms heterodimeric complexes by associating non-covalently with integrin alpha subunits: alphaV (CD51) to create the Vitronectin Receptor and alphaIIb (CD41) to form gpIIb/IIIa. These complexes are responsible for adhesion to extracellular matrix components, facilitating cell adhesion and cell-surface mediated signaling. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, and osteoclasts in association with CD51. The protein product of CD61 is composed of an alpha chain and a beta chain, which can combine with multiple partners to form different integrins. Its involvement in cell adhesion and signaling underscores its importance in normal physiological processes. Dysfunction of CD61 is associated with diseases such as Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder, highlighting its critical role in hemostasis and platelet function.

Specifications

• Antigen: CD61 (Integrin beta 3)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P05106
• Gene Symbols: ITGB3
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 102117791, 3690, 403788, 717379
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: VI-PL2
• Conjugate: Super Bright 645
• Gene: ITGB3
• Gene Alias: antigen CD61; BDPLT16; BDPLT2; beta3 integrin; CD61; EGK_08849; glycoprotein GPIIIa; glycoprotein IIIa; GP3A; GPIIIa; GT; INGRB3; integrin beta 3; integrin beta 3 (Cd61); integrin beta-3; integrin beta-3 subunit; integrin subunit beta 3; integrin, beta 3; integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61); ITGB3; platelet glycoprotein IIIa; platelet glycoprotein IIIa integrin subunit beta 3; platelet gpIIIa; platelet membrane glycoprotein IIIa
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Baboon, Canine, Cynomolgus Monkey, Human, Rhesus Macaque
• Product Type: Antibody
• Isotype: IgG1 κ