CD62E (E-selectin) Monoclonal Antibody (P2H3), PE, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-255-0894	Invitrogen 12062741	25 Tests
50-112-2242	Invitrogen 12062742	100 Tests

Description

Description: The P2H3 monoclonal antibody reacts with human CD62E, a 97-115 kDa member of the selectin family. CD62E, also known as E-selectin or endothelial-leukocyte adhesion molecule-1 (ELAM-1) is an adhesion molecule expressed by endothelial cells upon stimulation with cytokines including TNFalpha and IL-1beta. Induced expression of CD62E during inflammatory conditions is thought to mediate leukocyte rolling including the initial interaction of neutrophils with endothelium. The P2H3 monoclonal antibody also inhibits cellular adhesion to cytokine-activated endothelial cells. Applications Reported: This P2H3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This P2H3 antibody has been pre-titrated and tested by flow cytometric analysis of TNF alpha-actiavted Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

Specifications

• Antigen: CD62E (E-selectin)
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: CD62 antigen-like family member E; CD62E; e selectin; Elam; ELAM1; ELAM-1; endothelial adhesion molecule 1; endothelial leukocyte adhesion molecule; endothelial leukocyte adhesion molecule 1; endothelial leukocyte adhesion molecule I; ESEL; E-selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2; SELE; selectin E; selectin E (endothelial adhesion molecule 1); selectin, endothelial cell
• Gene Symbols: SELE
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: SELE

• Clone: P2H3
• Applications: Flow Cytometry
• Conjugate: PE
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P16581
• Gene ID (Entrez): 6401
• Isotype: IgG1
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.