CD64 Mouse anti-Human, Brilliant Ultraviolet 737, Clone: 10.1, BD Horizon™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB612777	BD Biosciences 612777	25 Tests
BDB612776	BD Biosciences 612776	100 Tests

Description

The 10.1 monoclonal antibody specifically binds to CD64, a 72 kDa type I transmembrane glycoprotein that is a high affinity receptor for human IgG (FcγRI), especially the IgG1 and IgG3 subclasses. CD64 is expressed on monocytes, macrophages, dendritic cells, granulocytes activated with interferon-gamma and early myeloid lineage cells. CD64 associates with a signaling FcRγhomodimer to form the functional high affinity FcγRI complex. CD64 functions in both innate and adaptive immune responses and mediates endocytosis, phagocytosis, antigen presentation, antibody-dependent cellular toxicity, cytokine release and superoxide generation.

Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV737 reagents (e.g., CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.

Specifications

• Antigen: CD64
• Applications: Flow Cytometry
• Conjugate: Brilliant Ultraviolet 737
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Isotype: IgG1 κ
• Purification Method: Affinity Purified

• Clone: 10.1
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Human
• Immunogen: Human Rheumatoid synovial fluid cells and fibronectin-purified monocytes
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.