CD8 Antibody (4B11), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 4 publications

Supplier:  Novus Biologicals NB10065729

Catalog No. NB10065729

Description

CD8 Monoclonal specifically detects CD8 in Human samples. It is validated for Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Dual RNAscope ISH-IHC.

Specifications

• Antigen: CD8
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Tissue culture supernatant with 0.09% Sodium Azide
• Gene Alias: CD8, CD8a molecule, LEU2, p32
• Host Species: Mouse
• Molecular Weight of Antigen: 26 kDa
• Quantity: 0.1 mL
• Research Discipline: Adaptive Immunity, Cytokine Research, Immunology, Innate Immunity, Signal Transduction, Stem Cell Markers
• Gene ID (Entrez): 925
• Target Species: Human
• Form: Supernatant

• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), In Situ Hybridization (ISH)
• Clone: 4B11
• Dilution: Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:20-1:40, Immunohistochemistry-Frozen 1:20-1:40, Dual RNAscope ISH-IHC
• Gene Accession No.: P01732
• Gene Symbols: CD8A
• Immunogen: Synthetic peptide derived from the carboxy terminal region of the humanCD8 alpha chain coupled to a N-terminal cysteine, with the sequence C-KSDGKPSLSARYV. The peptide was coupled to bovine serum albuman and keyhole limpet hemocyanin.
• Purification Method: Tissue culture supernatant
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes the human CD8 cell surface antigen, a 32kD glycoprotein expressed by the cytotoxic/suppressor subset of T-cells and weakly by NK cells. Clone 4B11 has been reported as being suitable for use in Western blotting.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG2b