CD8b Monoclonal Antibody (SIDI8BEE), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
14-527-337	Invitrogen™ 14527337	2 mg

Description

The SIDI8BEE MAb reacts with human and rhesus macaque CD8 beta. The CD8b chain associates with the CD8a chain to form the CD8ab heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. The CD8 receptor complex binds to MHC class I and plays a role in T cell development and activation of mature T cells. Long been thought to exist either as a heterodimer of the alpha and beta chain or as a homodimer of alpha chains, beta chains have now been shown to homodimerize as well. SIDI8BEE can detect the CD8 beta chain in both the CD8ab heterodimer and the CD8bb homodimer. SIDI8BEE is cross-reactive to human and rhesus macaque CD8b. Other Non-human Primate species have not been tested.

The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.

Specifications

• Antigen: CD8b
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene ID (Entrez): 926
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: Cd8b

• Clone: SIDI8BEE
• Applications: Flow Cytometry, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Monkey
• Gene Accession No.: P10966
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody