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CD99 Antibody (12E7 + MIC2/877), Novus Biologicals™
SDP

Catalog No. NBP24469701 Shop All R&D Systems Products
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Catalog No. NBP24469701 Supplier Novus Biologicals Supplier No. NBP2446970.1ML
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

CD99 Monoclonal specifically detects CD99 in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

Antigen CD99
Applications Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Classification Monoclonal
Clone 12E7 + MIC2/877
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry 5 - 10 ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1:100-1:200, Immunofluorescence 1:100-1:200
Formulation No buffer with 0.05% Sodium Azide
Gene Accession No. P14209, P14209
Gene Alias 12E7, antigen identified by monoclonal antibodies 12E7, F21 and O13, CD99 antigenY homolog, CD99 molecule, E2 antigen, HBA71, MIC2 (monoclonal 12E7), MIC2Y, MSK5X, Protein MIC2, surface antigen MIC2, T-cell surface glycoprotein E2
Gene Symbols CD99
Host Species Mouse
Immunogen Human acute lymphocytic leukemia T-cells (12E7); Recombinant human MIC2 protein (MIC2/877)
Purification Method Tissue culture supernatant
Quantity 0.1 mL
Regulatory Status RUO
Research Discipline Immunology
Primary or Secondary Primary
Gene ID (Entrez) 4267
Test Specificity Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewings sarcoma cells and primitive peripheral neuroectodermal tumors.
Target Species Human
Content And Storage Store at 4C.
Form Supernatant
Isotype IgG
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