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CD99 Antibody (SPM586), Novus Biologicals™
SDP

Catalog No. NBP24469300 Shop All R&D Systems Products
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NBP24469301 0.1 mg
NBP24469302 0.2 mg
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Catalog No. NBP24469300 Supplier Novus Biologicals Supplier No. NBP2446930.02MG
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

CD99 Monoclonal specifically detects CD99 in Human, Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.

Specifications

Antigen CD99
Applications Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Classification Monoclonal
Clone SPM586
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Flow Cytometry 5 - 10ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1 - 2ug/ml, Immunofluorescence 0.5 - 1.0ug/ml, CyTOF-ready
Formulation 10mM PBS with 0.05% Sodium Azide
Gene Alias 12E7, antigen identified by monoclonal antibodies 12E7, F21 and O13, CD99 antigenY homolog, CD99 molecule, E2 antigen, HBA71, MIC2 (monoclonal 12E7), MIC2Y, MSK5X, Protein MIC2, surface antigen MIC2, T-cell surface glycoprotein E2
Gene Symbols CD99
Host Species Mouse
Immunogen Purified E-rosette forming cells from human peripheral blood lymphocytes
Purification Method Protein A or G purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Immunology
Primary or Secondary Primary
Gene ID (Entrez) 4267
Test Specificity Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Target Species Human, Rat
Content And Storage Store at 4C.
Form Purified
Isotype IgM κ
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For Research Use Only

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