Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. K1639B

Thermo Scientific™ CellSensor™ DHFR/E2F-bla NIH3T3 Cell Line

Catalog No. K1639B
Encompass_Preferred
Change view
Click to view available options
Quantity:
1 Vial
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
K1639B 1 Vial
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. K1639B Supplier Thermo Scientific™ Supplier No. K1639B
Only null left
Add to Cart
Add to Cart
Request a Quote

Description

The CellSensor™ dhfr(E2F)-bla NIH 3T3 Cell Line contains a beta-lactamase reporter gene under control of the E2F/DP1 binding sequence from the DHFR gene promoter, stably integrated into NIH 3T3 cells.

The CellSensor™ dhfr(E2F)-bla NIH 3T3 Cell Line contains a beta-lactamase reporter gene under control of the E2F/DP1 binding sequence from the DHFR gene promoter, stably integrated into NIH 3T3 cells. This cell line is a clonal population isolated by flow cytometry in response to 10% newborn bovine serum. This cell line has been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time, and validated for Z-factor using newborn bovine serum. Additional data using alternate stimuli are shown. The G1/S cell-cycle checkpoint controls the passage of eukaryotic cells from the first gap phase (G1) into the DNA synthesis phase (S). Two cell-cycle kinases, CDK4/6-cyclin D and CDK2-cyclin E, and the transcription complex that includes Rb and E2F, are pivotal in controlling this checkpoint. During G1, the Rb-HDAC repressor complex binds to the E2F-DP1 transcription factors, inhibiting downstream transcription. Phosphorylation of Rb by CDK4/6 and CDK2 dissociates the Rb-repressor complex, permitting transcription of S-phase genes encoding for proteins that amplify the G1-to-S switch and that are required for DNA replication. Many different stimuli exert checkpoint control, including TGFβ, DNA damage, contact inhibition, replicative senescence, and growth factor withdrawal. Academic and non-profit customers, please inquire for special pricing.

TRUSTED_SUSTAINABILITY

Specifications

Cell State Dividing Cells
Content And Storage CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Quantity 1 Vial
System Type CellSensor™
Druggable Target Signaling Pathway

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.