Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Cell Analysis Products
  4. Cell Based Assays
  5. General Cell Based Assays
  6. K1838

Thermo Scientific™ CellSensor™ Gli-bla 22Rv.1 Cell Line

Catalog No. K1838
Encompass_Preferred
Change view
Click to view available options
Quantity:
1 vial
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
K1838 1 vial
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. K1838 Supplier Thermo Scientific™ Supplier No. K1838
Only null left
Add to Cart
Add to Cart
Request a Quote

Description

The morphogenic signal Shh provides in the developing CNS induces proliferation of neuronal precursor cells in the developing cerebellum and other tissues.

The morphogenic signal Shh provides in the developing CNS induces proliferation of neuronal precursor cells in the developing cerebellum and other tissues. Proliferative signaling by Shh is involved in the development of cancer, including specific brain and skin cancers such as basal cell carcinomas. Signaling takes place through a Patched (PTC-1)/Smoothened (SMO) Receptor complex. The activation of Patched by Shh releases the inhibition of Patched on Smoothened leading to Sonic Hedgehog pathway activation of the Gli transcription factor to induce downstream gene expression. The CellSensor™ Gli-bla 22Rv1 cell line contains a beta-lactamase reporter gene under control of the Gli response element stably integrated into 22Rv1 cells. This cell line is a clonal population isolated by flow cytometry. The clone was selected based on inhibition of the pathway with KAAD-Cyclopamine. This cell line has also been tested for assay performance under variable conditions, including DMSO concentration, cell number, compound incubation time, and substrate loading time and validated for Z' under maximal inhibition of β-lactamase activity with Clavulanic Acid.

TRUSTED_SUSTAINABILITY

Specifications

Antagonists KAAD-Cyclopamine
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage CellSensor™ Gli-bla 22Rv.1 cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Detection Method Fluorescence
For Use With (Application) FRET
Gene Symbol GLI
Human Protein Class Cell Signalling
Primary Agonist Constitutive
Product Line CellSensor
Quantity 1 vial
Readout End Point
Reporter Gene BLA (Beta-Lactamase), BLA (Beta-lactamase)
Target Entry SMO, Hedgehog pathway
Type Cell Line
Cell Line 22Rv.1
System Type CellSensor™
Druggable Target Signaling Pathway
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.