Invitrogen™ CellSensor™ Myc-bla HCT116 Cell Line

Description

CellSensor™ Myc-bla HCT116 Cell Line

The CellSensor™Myc-bla HCT116 cell line contains a beta-lactamase reporter gene under the control of Myc binding sequences. The construct was transduced into HCT116 cells using a lentiviral system. HCT116 is a colon cancer cell line which expresses a mutated form of beta-catenin. This form of beta-catenin leads to the accumulation of beta-catenin and constitutive activation of downstream genes such as Myc. This cell line is a clonal population isolated by flow cytometry. It has been validated for cell plating density and DMSO tolerance. The signaling pathway has been validated using RNAi against c-Myc and ICG-001, an inhibitor of the wnt-beta-catenin pathway. The expression of the mutant beta-catenin in HCT116 cells results in constitutive activation of beta-lactamase in this CellSensor™line, which can be knocked down by ICG-001 (Figure 1) or Myc RNAi (Figure 2).

Specifications

• Assay Entry: Cell-based beta lactamase reporter gene
• Cell State: Dividing Cells
• Content And Storage: CellSensor™ Myc-bla HCT116 cells are shipped on dry ice.
  Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
• Detection Method: Fluorescence
• Product Line: CellSensor™
• Quantity: 1 vial
• Target Entry: Myc pathway, MYC
• Type: Cell Line
• Cell Line: HCT-116
• System Type: CellSensor™
• Druggable Target: Signaling Pathway

For Research Use Only. Not for use in diagnostic procedures.