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Invitrogen™ CellSensor™ Myc-bla HCT116 Cell Line

Catalog No. K1467
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Description

CellSensor™ Myc-bla HCT116 Cell Line

The CellSensor™Myc-bla HCT116 cell line contains a beta-lactamase reporter gene under the control of Myc binding sequences. The construct was transduced into HCT116 cells using a lentiviral system. HCT116 is a colon cancer cell line which expresses a mutated form of beta-catenin. This form of beta-catenin leads to the accumulation of beta-catenin and constitutive activation of downstream genes such as Myc. This cell line is a clonal population isolated by flow cytometry. It has been validated for cell plating density and DMSO tolerance. The signaling pathway has been validated using RNAi against c-Myc and ICG-001, an inhibitor of the wnt-beta-catenin pathway. The expression of the mutant beta-catenin in HCT116 cells results in constitutive activation of beta-lactamase in this CellSensor™line, which can be knocked down by ICG-001 (Figure 1) or Myc RNAi (Figure 2).
TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage CellSensor™ Myc-bla HCT116 cells are shipped on dry ice.
Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Detection Method Fluorescence
Product Line CellSensor
Quantity 1 vial
Target Entry Myc pathway, MYC
Type Cell Line
Cell Line HCT-116
System Type CellSensor™
Druggable Target Signaling Pathway
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For Research Use Only. Not for use in diagnostic procedures.

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