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Invitrogen™ CellSensor™ NFKB-bla HEK293T Cell Line

Catalog No. K1664
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K1664 1 vial
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Catalog No. K1664 Supplier Invitrogen™ Supplier No. K1664
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Description

CellSensor™ NFKB-bla HEK293T cell line

The CellSensor™ NFκB-bla HEK 293T cell line contains a beta-lactamase reporter gene under the control of the Nuclear Factor kappa B (NFκB) response element. To obtain the cell line, the NFκB-bla construct was transduced into HEK 293T cells by lentivirus. Subsequent flow cytometry was used to isolate clones responsive to Tumor Necrosis Factor alpha (TNFα). The cell line has been validated for DMSO tolerance, stimulation time, substrate loading time, and EC50 concentration of TNFα. This cell line is responsive to TNFα and can be used to probe NFκB signaling pathways, including those that are involved in apoptosis, viral defense, cancer, inflammation, and autoimmune disease.
TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Product Line CellSensor
Quantity 1 vial
Target Entry TNF alpha, NIK, TLR, I kappa B, IRAK, IKK, NF-kappa B pathway
System Type CellSensor™
Druggable Target Signaling Pathway

For Research Use Only. Not for use in diagnostic procedures.

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