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Thermo Scientific™ CellSensor™ p53RE-bla HCT-116 Cell Line

Catalog No. K1640
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Catalog No. K1640 Supplier Thermo Scientific™ Supplier No. K1640

Please call customer service for current pricing. 1-800-766-7000

Description

The CellSensor™ p53RE-bla HCT-116 cell line contains a beta-lactamase reporter gene under control of the p53 response element stably integrated into HCT-116 cells.

The CellSensor™ p53RE-bla HCT-116 cell line contains a beta-lactamase reporter gene under control of the p53 response element stably integrated into HCT-116 cells. To construct this cell line, the p53RE-bla construct was transduced into HCT-116 cells by lentivirus. Flow cytometry was used to isolate cells responsive to Mitomycin. This cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading time, as well as Z'; and EC50 concentrations for Mitomycin. The CellSensor™ p53RE-bla HCT-116 cell line is responsive to Mitomycin and can be used to probe p53 signaling, which is involved in DNA repair, cell cycle arrest, and in some cases, apoptosis. This cell line can be adapted for high-throughput screening for agonists or antagonists with compound libraries. Candidate drugs can be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing.

TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Product Line CellSensor
Quantity 1 Vial
Target Entry p53, DNA damage/p53 response pathway
Type Cell Line
Cell Line HCT-116
System Type CellSensor™
Druggable Target Signaling Pathway

For Research Use Only. Not for use in diagnostic procedures.

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