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Thermo Scientific™ CellSensor™ T-REx™ FOXO3 DBE-bla HeLa Cell Line

Catalog No. K1468
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Description

The CellSensor™ T-REx™ FOXO3 DBE-bla HeLa cell line was engineered by lentiviral transduction of HeLa cervical cancer cells with a FOXO3 response element driving beta-lactamase expression, along with a tetracycline repressor and tetracycline (or the tetracycline analog doxycycline)-inducible FOXO3 constructs.

The CellSensor™ T-REx™ FOXO3 DBE-bla HeLa cell line was engineered by lentiviral transduction of HeLa cervical cancer cells with a FOXO3 response element driving beta-lactamase expression (DBE-bla), along with a tetracycline repressor and tetracycline (or the tetracycline analog doxycycline)-inducible FOXO3 constructs. Addition of doxycycline to these cells allows for FOXO3 transcription factor expression and subsequent beta-lactamase expression, which in turn can be down-regulated by insulin-mediated activation of the PI3K/AKT signaling pathway which leads to phosphorylation and inactivation of FOXO3 and concomitant suppression of beta-lactamase expression. This cell line has been tested for robust assay performance by assessing a variety of assay parameters, including: cell plating number, DMSO tolerance, stimulation time, and substrate loading time. Assay validation was performed using serial dilutions of insulin to antagonize doxycycline-induced FOXO3 activity (Figure 1). Additional testing data using RNAi and alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage CellSensor™ T-REx™ FOXO3 DBE-bla HeLa cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Detection Method Fluorescence
Product Line CellSensor, T-REx
Quantity 1 Vial
Target Entry IR, IGF1R, PI3K, AKT1, FOXO3, PI3K/AKT/FOXO3 pathway
Type Cell Line
Cell Line HeLa
System Type CellSensor™
Druggable Target Kinases
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For Research Use Only. Not for use in diagnostic procedures.

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