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Thermo Scientific™ CellSensor™ T-REx™ NICD CSL-bla HeLa Cell Line

Catalog No. K1487
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Catalog No. K1487 Supplier Thermo Scientific™ Supplier No. K1487
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Description

This cell line is a clonal population isolated by flow cytometry. Addition of doxycycline to these cells allows for regulated NICD transcription factor expression and subsequent beta-lactamase expression.

The CellSensor™ T-REx™ NICD CSL-bla HeLa cell line was engineered by lentiviral transduction of HeLa cervical cancer cells with a notchresponse element driving beta-lactamase reporter gene expression (CSL-bla) along with tetracycline repressor and tetracycline (or the tetracycline analog, doxycycline)-inducible NICD (notch intracellular domain) constructs. This cell line is a clonal population isolated by flow cytometry. Addition of doxycycline to these cells allows for regulated NICD transcription factor expression and subsequent beta-lactamase expression. This cell line has been tested for robust assay performance by assessing a variety of parameters, including cell plating number, DMSO tolerance, stimulation time, and substrate loading time. Assay validation was performed using serial dilutions of doxycycline. Additional testing data using RNAi and alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage CellSensor™ T-REx™ NICD CSL-bla HeLa cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Detection Method Fluorescence
Product Line CellSensor, T-REx
Quantity 1 Vial
Target Entry NICD, Notch pathway
Cell Line HeLa
System Type CellSensor™
Druggable Target Signaling Pathway

For Research Use Only. Not for use in diagnostic procedures.

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