CH25H Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 600401MM8S

Catalog No. 50-199-2329

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This affinity purified antibody is directed against human CH25H. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence and 92% homology to pig, 91% homology to rat, and 75% homology to mouse.

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.

Specifications

• Antigen: CH25H
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CH25H
• Gene Alias: 1.14.99.38; AI462618; BOS_23282; C25H; ch25h; ch25hb; Cholesterol 25-hydroxylase; cholesterol 25-hydroxylase-like; Cholesterol 25-hydroxylase-like protein; Cholesterol 25-monooxygenase; h25OH; LOC100341039; m25OH; zgc:101688
• Host Species: Rabbit
• Regulatory Status: RUO
• Gene ID (Entrez): 9023
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry, Western Blot
• Concentration: 0.92 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: O95992
• Gene Symbols: CH25H
• Quantity: 25 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody